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KMID : 1149220210290010001
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Korean Journal of Oriental Medical Prescription
2021 Volume.29 No. 1 p.1 ~ p.8
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Study of Ojayeonjonghwan on hydrogen peroxide-induced oxidative stress in male reproductive GC-1 germ cell lines
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Chang Mun-Seog
Lee Ho-Chul
Yi Seung-Ho
Park Seong-Kyu
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Abstract
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Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Ojayeonjonghwan (OYH) in GC-1 germ cell lines.
Methods : DPPH radical scavenging activity and cell viability assays in GC-1 germ cell lines were performed. In addition, the protective effects of OYH against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines were examined by measuring cell viability after H2O2 treatmet. The formation of ROS and the antioxidant enzymes activity such as SOD and catalase were measured in the same condition.
Results : OYH scavenged DPPH radical dose-dependent manner and the IC50 was 63.79 μg/ml. OYH showed no cytotoxicity at concentration of 1, 10, 100 μg/ml. The hydrogen peroxide-induced cytotoxicity of GC-1 germ cell lines was protected to 53.66% by OYH at concentration of 10 μg/ml. OYH effectively inhibited ROS production in GC-1 germ cell lines. Mn SOD and catalase protein expression were significantly increased in GC-1 germ cell lines, but Cu/Zn SOD protein expression was not significantly changed.
Conclusions : In conclusion, OYH has antioxidant activities against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines.
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KEYWORD
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water extract of Ojayeonjonghwan (OYH), GC-1 germ cell lines, cytotoxicity, MTT assay, superoxide dismutase (SOD), catalase activity
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